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Guide lines to set up PCR lab to avoid cross contaminationAuthor: Owais Yaqub

The most common contamination in the PCR laboratory is its own previously generated AMPLICONS and it is the potential source of invalid PCR results. A laboratory performing PCR analyses should be divided into at least three physically separate rooms which are as follows:

  • The Reagent preparation 
  • RoomSample preparation 
  • RoomAmplification Room

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